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Lactobacillus MRS broth (LMRS BROTH) is an enriched selective medium for the isolation and cultivation of Lactobacillus found in clinical, dairy, and food specimens. This medium is prepared, dispensed, and packaged under oxygen-free conditions to prevent the formation of oxidized products prior to use.Lactobacillus MRS broth is an enriched selective medium for the cultivation of Lactobacillus from clinical, dairy, and food specimens. LMRS broth consists of proteose peptone no. 3, beef extract, yeast extract, and dextrose as the nutritive base. The medium is supplemented with polysorbate 80 (Tween 80) and magnesium as a source of fatty acids and additional growth requirements. Sodium acetate and ammonium citrate may inhibit normal flora, such as gram-negative bacteria, oral flora, and fungi. Improved recovery of Lactobacilli occurs in the presence of these selective agents.